It is an ideal inspection tool for all. Magnification is one important parameter for determining the performance of an optical microscope. A report from Leica Microsystems published in the journal Microscopy Today clarifies guidelines that help users determine the useful range of magnification values for digital microscopy.
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Digital micros. The fully inte. The Leica DVM6 digit. All sciencelab 84 news 47 page 29 product 1 event. Since fluorescence emission has a lifetime in the nanosecond scale, continuous excitation is needed to collect an image having fluorescence as contrast mechanism. In a classical wide-field optical fluorescence microscope, the light source of a fluorescent microscope is a gas-vapor arc lamp, using either a discrete Mercury or a continuous Xenon light-emission spectrum.
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Continuous- or pulsed-light-emitting lasers are now used in modern fluorescence microscopy beam-scanning techniques, as confocal laser-scanning microscopy and multi-photon excitation microscopy. In the most common Skip to main content Skip to table of contents.
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Encyclopedia of Biophysics Edition. Contents Search. Optical Fluorescence Microscopy. How to cite. Basic Characteristics The wide-field fluorescence microscope is a modification of the compound optical microscope, arranged to admit light of a selected wavelength to fluorescently labeled specimens and to image them through the emitted radiation.
This is a preview of subscription content, log in to check access. Bradbury S, Bracegirdle B. Introduction to light microscopy. In: Royal microscopical society microscopy handbooks, vol Google Scholar.
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Diaspro A. Confocal and two-photon microscopy: foundations, applications, and advances. New York: Wiley-Liss; Diaspro A, editor.
Optical fluorescence microscopy: from the spectral to the nano dimension.