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It is an ideal inspection tool for all. Magnification is one important parameter for determining the performance of an optical microscope. A report from Leica Microsystems published in the journal Microscopy Today clarifies guidelines that help users determine the useful range of magnification values for digital microscopy.

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Digital micros. The fully inte. The Leica DVM6 digit. All sciencelab 84 news 47 page 29 product 1 event. Since fluorescence emission has a lifetime in the nanosecond scale, continuous excitation is needed to collect an image having fluorescence as contrast mechanism. In a classical wide-field optical fluorescence microscope, the light source of a fluorescent microscope is a gas-vapor arc lamp, using either a discrete Mercury or a continuous Xenon light-emission spectrum.


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Continuous- or pulsed-light-emitting lasers are now used in modern fluorescence microscopy beam-scanning techniques, as confocal laser-scanning microscopy and multi-photon excitation microscopy. In the most common Skip to main content Skip to table of contents.

Leica motCORR Objectives

Encyclopedia of Biophysics Edition. Contents Search. Optical Fluorescence Microscopy. How to cite. Basic Characteristics The wide-field fluorescence microscope is a modification of the compound optical microscope, arranged to admit light of a selected wavelength to fluorescently labeled specimens and to image them through the emitted radiation.

Fluorescence Microscopy Animation

This is a preview of subscription content, log in to check access. Bradbury S, Bracegirdle B. Introduction to light microscopy. In: Royal microscopical society microscopy handbooks, vol Google Scholar.

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Diaspro A. Confocal and two-photon microscopy: foundations, applications, and advances. New York: Wiley-Liss; Diaspro A, editor.

Optical fluorescence microscopy: from the spectral to the nano dimension.